Microscopy, Imaging, and Flow Cytometry Equipment

Microscopy, Imaging, and Flow Cytometry Equipment

Major Microscopy and Imaging Instruments

Leica Thunder Live Cell 3D microscope

Leica Thunder Live Cell 3D microscope (CC rm. 132A)

  1. TIRF and Photomanipulation Modules
  2. High power TIRF for super resolution single molecule localization microscopy
  3. Automated Leica DMI8 microscope with incubator for environmental control
  4. LED8 for epi-illumination, DIC and phase contrast capable
  5. Hamamatsu Flash 4.0 v3 camera
  6. Gemini camera mount for simultaneous 2-channel imaging (Em 520/40 and 605/70)
  7. Laser excitation wavelengths:  405, 488, 561, and 638 nm
  8. 350 nm Pulsed Laser Unit
  9. Objectives:  5x/0.12 PH0, 10x/0.32 PH1, 20x/0.40 corr PH1, 20x/0.80 oil DIC PLU, 63x/1.2 w corr uvis DIC cs2 PLU, 100x/1.47 oil corr DIC TIRF, 160x/1.43 TIRF/GSD
  10. Advanced LASX software features including:  THUNDER computation clearing, Navigator with tile scanning and multi-position experiments, 3D Analysis, and 3D Visualization Advanced

Please credit the National Institutes of Health Shared Instrument Grant program "NIH 1S10OD028515-01" when citing use of this instrument.



Leica TCS SP5 confocal microscope

Leica TCS SP5 confocal microscope (CC rm. 132A)

  1. Automated Leica DMI 6000 microscope with 2.5x/0.07 pl fl, 10x/0.3 hc pl fl, 40x/1.25 oil hcx pl apo cs, 63x/1.4 oil hcx pl apo cs objectives.
  2. Laser excitation wavelengths: 405, 458, 476, 488, 496, 514, 543, 594, and 633 nm
  3. Acousto-Optical Beam Splitter



MMI Laser capture microdissection system

MMI Laser capture microdissection system (CC rm. 132A)

  1. Automated Olympus IX81 microscope with 4xPhL/0.13 uplfln, 10xPh1/0.3 uplfln, 20xPh1/0.45 lucplfln (0-2), 40xPh2/0.6 lucplfln, and 60xPh2/0.7 objectives
  2. Laser cutting capabilities (MMI Cell Cut Plus) with mini caplift technology
  3. MMI CellEctor Plus capillary based sorting system for cells in suspension



Olympus time-lapse system

Olympus time-lapse system (CC rm. 132A)

  1. Automated Olympus IX81 microscope with 4x/0.13 uplfln, 10x/0.3 uplfln, 20xPh1/0.5 uplfln, 40x/0.95 uplsapo (0.11-0.23) and 60x/1.35 oil uplsapo objectives.
  2. Environmental chamber with CO2, O2, and temperature control



Xenogen IVIS Lumina

Xenogen IVIS Lumina (BSB Vivarium)

  1. Fluorescence /luminescence in vivo/ in vitro imaging
  2. Sensitive detection of low emission light

Epifluorescence Microscopes

Olympus IX71 microscope
Olympus IX71 microscope

Olympus IX71 microscope (CC rm. 132)

  1. Inverted microscope with 10xPh1/0.3 uplfln, 20xPh1/0.45 lucplfln, 40xPh2/0.6 lucplfln, 60x/1.35 oil uplapo objectives
  2. Q imaging Retiga 2000R CCD monochrome and MF603C 6MP CCD color cameras








Zeiss Axioplan microscope
Zeiss Axioplan microscope

Zeiss Axioplan microscope (CC rm. 132)

  1. Upright microscope with 10xPh1/0.3 pl nf, 20xPh2/0.5 pl nf, 40xPh2/0.75 pl nf, 63x/1.4 oil pl apo, and 100x/1.3 oil pl nf objectives
  2. Coolsnap CCD color camera



Nikon Diaphot microscope
Nikon Diaphot microscope

Nikon Diaphot microscope (CC rm. 132)

  1. Inverted microscope with 10xPh1/0.3 pl nf, 20xPh2/0.5 pl nf, 40xPh2/0.75 pl nf, 63x/1.4 oil pl apo, and 100x/1.3 oil pl nf
  2. Microfire CCD color camera

Flow Cytometers

BD Accuri C6 flow cytometer
BD Accuri C6 flow cytometer

BD Accuri C6 flow cytometer (CC rm. 132)

  1. 2-laser-4-color analyzer
  2. 488 nm and 640 nm laser lines
  3. C-sampler for autosampling from 48- and 96-well plates



BD LSR Fortessa flow cytometer
BD LSR Fortessa flow cytometer

BD LSR Fortessa flow cytometer (BSB rm. 330)

  1. 4-laser-16 color analyzer
  2. 405, 488, 561, and 640 nm laser lines
  3. HTS for high-throughput sampling from 96-well plates

Imaging and Data Analysis Software

  1. Metamorph ( processing of microscopy images)
  2. FlowJo (processing of flow cytometry data)