Microscopy, Imaging, and Flow Cytometry Equipment
Microscopy, Imaging, and Flow Cytometry Equipment
Major Microscopy and Imaging Instruments
Leica Thunder Live Cell 3D microscope (CC rm. 132A)
- TIRF and Photomanipulation Modules
- High power TIRF for super resolution single molecule localization microscopy
- Automated Leica DMI8 microscope with incubator for environmental control
- LED8 for epi-illumination, DIC and phase contrast capable
- Hamamatsu Flash 4.0 v3 camera
- Gemini camera mount for simultaneous 2-channel imaging (Em 520/40 and 605/70)
- Laser excitation wavelengths: 405, 488, 561, and 638 nm
- 350 nm Pulsed Laser Unit
- Objectives: 5x/0.12 PH0, 10x/0.32 PH1, 20x/0.40 corr PH1, 20x/0.80 oil DIC PLU, 63x/1.2 w corr uvis DIC cs2 PLU, 100x/1.47 oil corr DIC TIRF, 160x/1.43 TIRF/GSD
- Advanced LASX software features including: THUNDER computation clearing, Navigator with tile scanning and multi-position experiments, 3D Analysis, and 3D Visualization Advanced
Please credit the National Institutes of Health Shared Instrument Grant program "NIH 1S10OD028515-01" when citing use of this instrument.
Leica TCS SP5 confocal microscope (CC rm. 132A)
- Automated Leica DMI 6000 microscope with 2.5x/0.07 pl fl, 10x/0.3 hc pl fl, 40x/1.25 oil hcx pl apo cs, 63x/1.4 oil hcx pl apo cs objectives.
- Laser excitation wavelengths: 405, 458, 476, 488, 496, 514, 543, 594, and 633 nm
- Acousto-Optical Beam Splitter
MMI Laser capture microdissection system (CC rm. 132A)
- Automated Olympus IX81 microscope with 4xPhL/0.13 uplfln, 10xPh1/0.3 uplfln, 20xPh1/0.45 lucplfln (0-2), 40xPh2/0.6 lucplfln, and 60xPh2/0.7 objectives
- Laser cutting capabilities (MMI Cell Cut Plus) with mini caplift technology
- MMI CellEctor Plus capillary based sorting system for cells in suspension
Olympus time-lapse system (CC rm. 132A)
- Automated Olympus IX81 microscope with 4x/0.13 uplfln, 10x/0.3 uplfln, 20xPh1/0.5 uplfln, 40x/0.95 uplsapo (0.11-0.23) and 60x/1.35 oil uplsapo objectives.
- Environmental chamber with CO2, O2, and temperature control
Xenogen IVIS Lumina (BSB Vivarium)
- Fluorescence /luminescence in vivo/ in vitro imaging
- Sensitive detection of low emission light
Epifluorescence Microscopes

Olympus IX71 microscope (CC rm. 132)
- Inverted microscope with 10xPh1/0.3 uplfln, 20xPh1/0.45 lucplfln, 40xPh2/0.6 lucplfln, 60x/1.35 oil uplapo objectives
- Q imaging Retiga 2000R CCD monochrome and MF603C 6MP CCD color cameras

Zeiss Axioplan microscope (CC rm. 132)
- Upright microscope with 10xPh1/0.3 pl nf, 20xPh2/0.5 pl nf, 40xPh2/0.75 pl nf, 63x/1.4 oil pl apo, and 100x/1.3 oil pl nf objectives
- Coolsnap CCD color camera

Nikon Diaphot microscope (CC rm. 132)
- Inverted microscope with 10xPh1/0.3 pl nf, 20xPh2/0.5 pl nf, 40xPh2/0.75 pl nf, 63x/1.4 oil pl apo, and 100x/1.3 oil pl nf
- Microfire CCD color camera
Flow Cytometers

BD Accuri C6 flow cytometer (CC rm. 132)
- 2-laser-4-color analyzer
- 488 nm and 640 nm laser lines
- C-sampler for autosampling from 48- and 96-well plates

BD LSR Fortessa flow cytometer (BSB rm. 330)
- 4-laser-16 color analyzer
- 405, 488, 561, and 640 nm laser lines
- HTS for high-throughput sampling from 96-well plates
Imaging and Data Analysis Software
- Metamorph ( processing of microscopy images)
- FlowJo (processing of flow cytometry data)