University of Hawai‘i at Manoa Homepage

Microscopy, Imaging and Flow Cytometry

The Microscopy, Imaging, and Flow Cytometry Shared Resource was established to fulfill a critical need for Cancer Center investigators whose research involves the imaging of biological systems from gross anatomical to subcellular levels. The state-of-the-art instrumentation made available by this resource enables researchers to analyze or process their samples in a wide variety of ways, including high resolution optical sectioning of fixed cells and tissues, time-lapse imaging of living cell cultures, non-invasive monitoring of tumor formation in preclinical studies, and extracting specific cells of interest from tissue samples. This resource also has two flow cytometers available to enable researches to analyze suspensions of cells for their differences in protein expression, gene expression, or cell morphology. In addition we offer technical training to users and are responsible for maintaining the equipment through preventative maintenance services, routine calibrations, upgrades, and repairs. It is our continuing mission to facilitate the use of the current instruments and pursue the acquisition of new instrumentation in accordance with the needs of the Cancer Center researchers. For more information about the facility's current instrumentation and software, please see the details listed below. For information on instrument availability, reservations, and rates, please contact the facility's director or research coordinator.

Major Microscopy and Imaging Instruments

  • Leica  TCS SP5 confocal microscope (CC rm. 132A)
    • Automated Leica DMI 6000 microscope with 2.5x, 10x, 40x, 63x objectives.
    • Laser excitation wavelengths: 405, 458, 476, 488, 496, 514, 543, 594, and 633 nm
  • Laser  microdissection system (CC  rm. 132A)
    • Automated Olympus IX81 microscope with 4x, 10x, 20x, 40x, and 60x objectives
    • Laser cutting capabilities (MMI Cell Cut Plus) with mini caplift technology
  • Olympus time-lapse system (CC  rm. 132A)
    • Automated Olympus IX81 microscope with 4x, 10x, 20x, and 60x objectives.
    • Environmental chamber with CO2 and temperature control
  • Xenogen IVIS Lumina (BSB Vivarium)
    • Fluorescence /luminescence in vivo/ in vitro imaging
    • Sensitive detection of low emission light

Epifluorescence Microscopes

  • Olympus IX71 microscope (CC  rm. 132)
    • Inverted microscope with 10x, 20x, 40x, 63x, and 100x objectives; 3 color filter set
    • Q imaging CCD monochrome camera
  • Zeiss Axioplan microscope (CC  rm. 132)
    • Upright microscope with 10x, 20x, 40x, 60x, and 100x objectives; 3 color filter set
    • Coolsnap CCD color camera
  • Nikon Diaphot microscope (CC  rm. 132)
    • Inverted microscope with 2.5x, 20x, 40x dry objectives; B-2A filter set
    • Microfire CCD color camera

Flow Cytometers

  • BD Accuri C6 flow cytometer (CC rm. 132)
    • 2-laser-4-color analyser
    • 488 nm and 640 nm laser lines
    • Accommodates tubes or welled plates
  • BD LSR Fortessa flow cytometer (BSB rm. 330)
    • 4-laser-12-color analyser
    • 405, 488, 561, and 640 nm laser lines
    • Accommodates tubes or 96 well plates

Imaging and Data Analysis Software

  • Metamorph ( processing of microscopy images)
  • Leica LAS (processing of confocal images)
  • FlowJo (processing of flow cytometry data)